Data from: Reconstruction of genetically identified neurons imaged by serial-section electron microscopy

Resolving patterns of synaptic connectivity in neural circuits currently requires serial section electron microscopy. However, complete circuit reconstruction is prohibitively slow and may not be necessary for many purposes such as comparing neuronal structure and connectivity among multiple animals. Here, we present an alternative strategy, targeted reconstruction of specific neuronal types. We used viral vectors to deliver peroxidase derivatives, which catalyze production of an electron-dense tracer, to genetically identified neurons, and developed a protocol that enhances the electron-density of the labeled cells and while retaining quality of the ultrastructure. The high contrast of the marked neurons enabled two innovations that dramatically speed data acquisition: targeted high-resolution reimaging of regions selected from rapidly-acquired lower resolution reconstruction, and an unsupervised segmentation algorithm. This pipeline reduces imaging and reconstruction times by at least two orders of magnitude, facilitating directed inquiry of circuit motifs.Joesch et al. 2016 - Data Fig. 3k - sections 205-231Joesch et al. 2006 - Fig 3k - sections 205-231.zipJoesch et al. 2016 - Fig 3k - sections 232-260Joesch et al. 2006 - Fig 3k - sections 232-260.zipJoesch et al. 2016 - Fig 3k - sections 261-280Joesch et al. 2006 - Fig 3k - sections 261-280.zipJoesch et al. 2016 - Fig 3k - sections 281-300Joesch et al. 2006 - Fig 3k - sections 281-300.zipJoesch et al. 2016 - Fig 3k - sections 301-320Joesch et al. 2006 - Fig 3k - sections 301-320.zipJoesch et al. 2016 - Fig 3k - sections 346-370Joesch et al. 2006 - Fig 3k - sections 346-370.zipJoesch et al. 2016 - Fig 3k - sections 321-345Joesch et al. 2006 - Fig 3k - sections 321-345.zipJoesch et al. 2016 - Fig 3k - sections 371-400Joesch et al. 2006 - Fig 3k - sections 371-400.zipJoesch et al. 2016 - Fig 3k - sections 426-450Joesch et al. 2006 - Fig 3k - sections 426-450.zipJoesch et al. 2016 - Fig 3k - sections 401-425Joesch et al. 2006 - Fig 3k - sections 401-425.zipJoesch et al. 2016 - Fig 3k - sections 451-475Joesch et al. 2006 - Fig 3k - sections 451-475.zipJoesch et al. 2016 - Fig 3k - sections 576-504Joesch et al. 2006 - Fig 3k - sections 576-504.zipOverview_Volume_Sections_205-504File InformationReadme file.Zip File Informationread m