Fig. 4. HPLC analysis of CYP76B100 and CYP76B101 microsomal enzyme assays in vitro incubated with GHQ. Microsomal preparation containing AtCPR1 alone was used as a negative control (black line). Microsomal proteins were respectively prepared from the engineered yeast strain co-expressing CYP76B100 and AtCPR1 (green line), and the engineered yeast strain co-expressing CYP76B101 and AtCPR1 (blue line).Published as part of Song, Wan, Zhuang, Yibin & Liu, Tao, 2020, Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis, pp. 1-9 in Phytochemistry (112375) 175 on page 4, DOI: 10.1016/j.phytochem.2020.112375, http://zenodo.org/record/829528
Fig. 4. Gas chromatography-mass spectrometry (GC-MS) analysis of the products in yeast strains harbo...
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversi...
Fig. 2. Hierarchical clustering of RNA-Seq expression data and heat map depicting the expression pro...
Fig. 2. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B100 in feeding exper...
Fig. 3. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B101 in feeding exper...
Fig. 1. The proposed biosynthetic pathway of shikonin in L. erythrorhizon. Single arrows represent o...
Fig. 5. Multiple amino acid sequence alignment of geranylhydroquinone 3″-oxygenases. Alignments of c...
Shikonin derivatives are specialized lipophilic metabolites, secreted in abundant amounts from the r...
Cytochrome P450s constitute the largest family of enzymatic proteins in plants acting on various end...
Cytochromes P450 (CYPs) play key role in generating the structural diversity of terpenoids, the larg...
Fig. 4. Identification of P. crispum SDH products by reversed-phase liquid chromatography coupled to...
Abstract: Geosmin is a terpenoid, an earthy-smelling substance associated with off-flavors in water ...
Plants possess very large numbers of biosynthetic cytochrome P450 enzymes. In spite of the importanc...
International audienceBackground: Large proliferations of cytochrome P450 encoding genes resulting f...
Plants produce an amazing diversity of specialized (secondary) metabolites, which in turn may underp...
Fig. 4. Gas chromatography-mass spectrometry (GC-MS) analysis of the products in yeast strains harbo...
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversi...
Fig. 2. Hierarchical clustering of RNA-Seq expression data and heat map depicting the expression pro...
Fig. 2. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B100 in feeding exper...
Fig. 3. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B101 in feeding exper...
Fig. 1. The proposed biosynthetic pathway of shikonin in L. erythrorhizon. Single arrows represent o...
Fig. 5. Multiple amino acid sequence alignment of geranylhydroquinone 3″-oxygenases. Alignments of c...
Shikonin derivatives are specialized lipophilic metabolites, secreted in abundant amounts from the r...
Cytochrome P450s constitute the largest family of enzymatic proteins in plants acting on various end...
Cytochromes P450 (CYPs) play key role in generating the structural diversity of terpenoids, the larg...
Fig. 4. Identification of P. crispum SDH products by reversed-phase liquid chromatography coupled to...
Abstract: Geosmin is a terpenoid, an earthy-smelling substance associated with off-flavors in water ...
Plants possess very large numbers of biosynthetic cytochrome P450 enzymes. In spite of the importanc...
International audienceBackground: Large proliferations of cytochrome P450 encoding genes resulting f...
Plants produce an amazing diversity of specialized (secondary) metabolites, which in turn may underp...
Fig. 4. Gas chromatography-mass spectrometry (GC-MS) analysis of the products in yeast strains harbo...
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversi...
Fig. 2. Hierarchical clustering of RNA-Seq expression data and heat map depicting the expression pro...