Fig. 2 in In silico characterization of class II plant defensins from Arabidopsis thaliana

Fig. 2. Alignment between sequences obtained at the end of semi-automatic search and known class II plant defensins. Signal peptide, mature defensin and Cterminal prodomain regions are indicated by the bottom ruler. The "GXC" γ-core motif is highlighted by a magenta box; none of A. thaliana sequences have the characteristic γ-core GXCX3-9C motif between the IV and VI cysteine residues. Cysteine residues are highlighted in yellow and the lines above the sequences indicate the bond pattern between them. Cis-proline motifs are marked in grey. Predicted C-terminal prodomain recognition site is highlighted by a green box, comprising a pair of charged residues, of which the first is always a negative one. Based on this signature, we propose the RegEx "CX8-10CX2-18CX3CX2-10[GAPSIDERYW]XCX4- 17CXCX3CX2[DE][DERK]" for future studies regarding the identification of class II defensins. Charged residues of C-Terminal prodomain are highlighted in blue (positively charged) or red (negatively charged). ZmERS6, NaD1, PhD1, PhD2 presented a negatively charged C-terminal prodomain, in contrast to A7REG2 (positively charged) and A7REG4 (neutral). Sd5 is a partial sequence, and despite the known terminal residues indicated a positively charged C-terminal prodomain, its actual charge is unknown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)Published as part of Costa, Laura S.M., Allan, Pires, S., Damaceno, Neila B., Rigueiras, Pietra O., Maximiano, Mariana R., Franco, Octavio L. & Porto, William F., 2020, In silico characterization of class II plant defensins from Arabidopsis thaliana, pp. 1-8 in Phytochemistry (112511) 179 on page 3, DOI: 10.1016/j.phytochem.2020.112511, http://zenodo.org/record/829325