CRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens. However, recent work has raised concerns regarding the performance of CRISPR-Cas9 screens using TP53 wild-type human cells due to a p53-mediated DNA damage response (DDR) limiting the efficiency of generating viable edited cells. To directly assess the impact of cellular p53 status on CRISPR-Cas9 screen performance, we carried out parallel CRISPR-Cas9 screens in wild-type and TP53 knockout human retinal pigment epithelial cells using a focused dual guide RNA library targeting 852 DDR-associated genes. Our work demonstrates that although functional p53 status negatively affects identification of significantly depleted genes, optimal screen design ca...
Summary: Human pluripotent stem cells (hPSCs) generate a variety of disease-relevant cells that can ...
RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic...
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats – Cas9) is an innovative tech...
AbstractCRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens...
Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and ce...
The recent developments of the CRISPR/Cas9 gene-editing system have made way for large-scale, loss-o...
The CRISPR/Cas9 system has emerged as a disruptive new tool to systematically probe gene function, b...
As a powerful genome-editing tool, the clustered regularly interspaced short palindromic repeats (CR...
Components of the type II CRISPR-Cas complex in bacteria have been used successfully in eukaryotic c...
Haapaniemi et al address the issues raised by Brown et al and discuss several differences between th...
The ability to systematically disrupt genes serves as a powerful tool for understanding their functi...
Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here we show t...
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic el...
Aims: Gene dosage can have a major impact on cell biology although, hitherto, is has been difficult ...
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 system for gen...
Summary: Human pluripotent stem cells (hPSCs) generate a variety of disease-relevant cells that can ...
RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic...
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats – Cas9) is an innovative tech...
AbstractCRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens...
Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and ce...
The recent developments of the CRISPR/Cas9 gene-editing system have made way for large-scale, loss-o...
The CRISPR/Cas9 system has emerged as a disruptive new tool to systematically probe gene function, b...
As a powerful genome-editing tool, the clustered regularly interspaced short palindromic repeats (CR...
Components of the type II CRISPR-Cas complex in bacteria have been used successfully in eukaryotic c...
Haapaniemi et al address the issues raised by Brown et al and discuss several differences between th...
The ability to systematically disrupt genes serves as a powerful tool for understanding their functi...
Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here we show t...
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic el...
Aims: Gene dosage can have a major impact on cell biology although, hitherto, is has been difficult ...
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 system for gen...
Summary: Human pluripotent stem cells (hPSCs) generate a variety of disease-relevant cells that can ...
RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic...
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats – Cas9) is an innovative tech...