Fig. 3 in Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry

Fig. 3. The LC/MS/MS fragmentation pattern from a 68-million-year-old T. rex peptide. (A) The experimental MS/MS spectrum for the T. rex doubly charged hydroxylated tryptic peptide sequence GVQPP(OH)GPQGPR from femur bone extract identified by LC/MS/MS. (B) The synthetic version of the same sequence. All major fragment ions from the experimental spectrum are in very good alignment with ions from the synthetic version, confirming the sequence. This molecular sequencing evidence of protein from a 68-million-year-old fossilized bone demonstrates excellent preservation of the T. rex femur and the high sensitivity of state-of-the-art MS technology.Published as part of John M. Asara, Mary H. Schweitzer, Lisa M. Freimark, Matthew Phillips & Lewis C. Cantley, 2007, Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry, pp. 280-284 in Science 316 (5822) on page 283, DOI: 10.1126/science.1137614, http://zenodo.org/record/374443