Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions

This data corresponds to the following paper: Title: Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions DOI: 10.1126/sciadv.add3643 Journal: Science Advances Authors: Josh P. Peters, Arne Brahms, Vivian Janicaud, Maria Anikeeva, Eva Peschke, Frowin Ellermann, Arianna Ferrari, Dana Hellmold, Janka Held-Feindt, Na-mi Kim, Johannes Meiser, Konrad Aden, Rainer Herges, Jan-Bernd Hövener, Andrey N. Pravdivtsev In the upload the figures 2 - 6 are covered, so all figures from the main manuscript with acquired data. Original abstract for the manuscript: Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times enabling in vivo metabolic imaging can be performed non-invasively in real-time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1-15N-NAM and 1-15N nicotinic acid and hyperpolarized them with dDNP, reaching (13.0±1.9)% 15N polarization. We found that the lifetime of hyperpolarized 1-15N-NAM is strongly field- and pH-dependent, with T1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1-15N lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using 15N dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a 15N MRI of 1-15N-NAM (previously hyperpolarized for more than an hour) in less than one second.We acknowledge funding from German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (01ZX1915C), DFG (PR 1868/3-1, HO-4602/2-2, HO-4602/3, GRK2154-2019, EXC2167, FOR5042, TRR287) and financial support by Land Schleswig-Holstein within the funding programme Open Access Publikationsfonds. MOIN CC was founded by a grant from the European Regional Development Fund (ERDF) and the Zukunftsprogramm Wirtschaft of Schleswig-Holstein (Project no. 122-09-053). AP acknowledges Kiel University PMI cluster of excellence for intramural supporting enzymatic LDH activity studies. JM received funding from FNR-INTER (NTER/BMBF/18/13399110). We are also thankful to Nicole Kiweler and Laura Neises from LIH for providing us with K-562 cells