Data from: Ecological adaptation and reproductive isolation in sympatry: genetic and phenotypic evidence for native host races of Rhagoletis pomonella

Ecological speciation-with-gene-flow may be an important mode of diversification for phytophagous insects. The recent shift of Rhagoletis pomonella from its native host downy hawthorn (Crataegus mollis) to introduced apple (Malus domestica) in the northeastern United States is a classic example of sympatric host race formation. Here, we test whether R. pomonella has similarly formed host races on four native Crataegus species in the southern United States: western mayhaw (C. opaca), blueberry hawthorn (C. brachyacantha), southern red hawthorn (C. mollis var. texana), and green hawthorn (C. viridis). These four southern hosts differ from each other in their fruiting phenology and in the volatile compounds emitted from the surface of their fruits. These two traits form the basis of ecological reproductive isolation between downy hawthorn and apple flies in the North. We report evidence from microsatellite population surveys and eclosion studies supporting the existence of genetically differentiated and partially reproductively isolated host races of southern hawthorn flies. The results provide an example of host shifting and ecological divergence involving native plants and imply that speciation-with-gene-flow may be commonly initiated in Rhagoletis when ecological opportunity presents itself.Table S5 - microsatellite allele frequenciesMicrosatellite allele frequencies for the 26 loci analyzed in study for 16 field sites, including 6 green hawthorn, 4 western mayhaw, 3 blueberry hawthorn, and 3 southern red hawthorn populations. See Table 1 of text for site descriptions. Allele numbers represent the size (in base pairs) of the PCR amplified DNA fragment (including the repeat sequence) generated using locus specific primers for each microsatellite, as determined by capillary electrophoresis on a Beckman-Coulter CEQ8000.Powell_etal_TableS5_for_DRYAD.xlsxmtDNA sequence alignmentClustal alignment of mtDNA sequences used in Figure 3. See Table S1 for accession numbers.Powell_etal_mtDNA_alignment.txtEclosion dataTable of laboratory eclosion data depicted in Figure 6.Powell_etal_eclosion_data.xls