Fig. 7. Flow cytometer analysis of LB accumulation in yeast cells. (A) Cytogram of BODIPY fluorescence vs. side scatter of cells expressing forage sorghum SbDGAT1- 1 variants. (B) Mean intensity of P3/fluorescent population (represents BODIPY uptake) events transformed with different variants of forage sorghum SbDGAT1-1 genes. The error bars represent the SD of three biological replicates. Asterisks indicates significant differences according to student t-test results where * = p <0.05.Published as part of Chawla, Kirti, Sinha, Kshitija, Neelam, Kaur, Ranjeet & Bhunia, Rupam Kumar, 2020, Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo,...
Diacylglycerol acyltransferases (DGATs) catalyze the final and only committed step of triacylglycero...
Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in it...
Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 ...
Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy....
Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic re...
Fig. 3. DISOPRED plot representing the intrinsically disordered regions in (A) SbDGAT1-1 (B) SbDGAT1...
Fig. 4. Transcript expression patterns of SbDGAT1-1 and SbDGAT1-2 in (A) bran (B) embryo (C) endospe...
Chawla, Kirti, Sinha, Kshitija, Neelam, Kaur, Ranjeet, Bhunia, Rupam Kumar (2020): Identification an...
Fig. 2. Sequence alignment of SbDGAT1-1 (XP_002439419) and SbDGAT1-2 (XP_021304830) deduced amino ac...
Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 protein...
Triacylglycerol (TAG) biosynthesis is a principal metabolic pathway in most organisms, and TAG is th...
<p>Type I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses ...
Fig. 3. Leaf characteristics of S. bicolor, S. brachypodum and S. macrospermum plants at six harvest...
RT-PCR detection of DGAT1 genes in transgenic yeast (INVSc1). The yeast actin was used as an interna...
<p>The initial step in glycerolipid biosynthesis, especially in diverse allopolyploid crop species, ...
Diacylglycerol acyltransferases (DGATs) catalyze the final and only committed step of triacylglycero...
Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in it...
Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 ...
Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy....
Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic re...
Fig. 3. DISOPRED plot representing the intrinsically disordered regions in (A) SbDGAT1-1 (B) SbDGAT1...
Fig. 4. Transcript expression patterns of SbDGAT1-1 and SbDGAT1-2 in (A) bran (B) embryo (C) endospe...
Chawla, Kirti, Sinha, Kshitija, Neelam, Kaur, Ranjeet, Bhunia, Rupam Kumar (2020): Identification an...
Fig. 2. Sequence alignment of SbDGAT1-1 (XP_002439419) and SbDGAT1-2 (XP_021304830) deduced amino ac...
Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 protein...
Triacylglycerol (TAG) biosynthesis is a principal metabolic pathway in most organisms, and TAG is th...
<p>Type I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses ...
Fig. 3. Leaf characteristics of S. bicolor, S. brachypodum and S. macrospermum plants at six harvest...
RT-PCR detection of DGAT1 genes in transgenic yeast (INVSc1). The yeast actin was used as an interna...
<p>The initial step in glycerolipid biosynthesis, especially in diverse allopolyploid crop species, ...
Diacylglycerol acyltransferases (DGATs) catalyze the final and only committed step of triacylglycero...
Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in it...
Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 ...