A COMPREHENSIVE STUDY ON BIOCHEMICAL ANALYSIS AND FREQUENCY OF VITAMIN D DEFICIENT PEOPLE Dr.

Objectives of the study: The basic objective of the study is to analyze the biochemical profile and frequency of vitamin D deficient people. Methodology of the study: This cross sectional study was conducted in Nishtar Hospital Multan during February 2018 to November 2018. The data was collected from 100 vitamin D deficient people who visited the OPD of the hospital regularly. One blood sample of 5ml was obtained from each patient. Blood tests were performed in the hospital laboratory. Serum 25- Hydroxycholecalciferol levels were determined by using a chemiluminesence assay. Serum Calcium, Phosphorus and alkaline phosphate were measured using an end point assay in a multi-channel analyzer. Intact PTH was measured using a 2-site chemiluminesence immunoassay. Samples were analyzed in multiple assays. Results: The data was collected from 100 patients of both genders. We compared the demographic variables of our sample in terms of age and gender. Although, there was no statistical difference between gender predisposition and Vitamin D deficiency, P-value was statistically significant for different age groups (p-value = 0.00). Among the biochemical markers which included Serum Calcium, Serum Phosphate, Serum Alkaline Phosphate and Serum Parathyroid Hormone, only Serum Phosphate showed statistically significant relationship (p-value = 0.000) with Vitamin D levels. Conclusion: It is concluded that Vitamin D deficiency is very common in Pakistan there is no any protocol for proper supplementation. Vitamin D deficiency in Pakistan is becoming endemic and it is much predominant in elder and female population