DETECTION OF HEPATITIS ANTIGEN AND ANTIBODY IN SERUM OF FEMALE HUMAN POPULATION

Immunization with hepatitis B (HB) vaccine is highly effective; however, more needs to be learned about the duration of protection and the need of booster dose. Present study suggest that age of vaccination is very important also it is observed that there is waning effect of vaccine after a long period. Our study throw light on the reliability of qualitative and quantitative tests to observe seropositivity of HBs antibodies. Qualitative detection of HBsAg was done with the help of chromatographic Hepacard method. For HBsAb detection we have done Immunochromatographic assay (Westernblotting). In order to monitor successful vaccination, chemiluminescent method is used to determine quantitatively HBsAb in the serum. The chromatographic methods are sensitive for the presence of antibody titer in serum below 100 mIU/ml. Therefore Enzyme linked chemiluminiscent method is very sensitive for quantitative determination of HBsAb titre and thereby informative for the requirement of booster dose of vaccination