Data from: Development of diagnostic microsatellite markers from whole-genome sequences of Ammodramus sparrows for assessing admixture in a hybrid zone

Studies of hybridization and introgression and, in particular, the identification of admixed individuals in natural populations benefit from the use of diagnostic genetic markers that reliably differentiate pure species from each other and their hybrid forms. Such diagnostic markers are often infrequent in the genomes of closely related species, and genomewide data facilitate their discovery. We used whole-genome data from Illumina HiSeqS2000 sequencing of two recently diverged (600,000 years) and hybridizing, avian, sister species, the Saltmarsh (Ammodramus caudacutus) and Nelson's (A. nelsoni) Sparrow, to develop a suite of diagnostic markers for high-resolution identification of pure and admixed individuals. We compared the microsatellite repeat regions identified in the genomes of the two species and selected a subset of 37 loci that differed between the species in repeat number. We screened these loci on 12 pure individuals of each species and report on the 34 that successfully amplified. From these, we developed a panel of the 12 most diagnostic loci, which we evaluated on 96 individuals, including individuals from both allopatric populations and sympatric individuals from the hybrid zone. Using simulations, we evaluated the power of the marker panel for accurate assignments of individuals to their appropriate pure species and hybrid genotypic classes (F1, F2, and backcrosses). The markers proved highly informative for species discrimination and had high accuracy for classifying admixed individuals into their genotypic classes. These markers will aid future investigations of introgressive hybridization in this system and aid conservation efforts aimed at monitoring and preserving pure species. Our approach is transferable to other study systems consisting of closely related and incipient species.SparrowGenotypesGenotypes at 12 diagnostic microsatellite loci of 96 Ammodramus caudacutus and A. nelsoni. Sparrows were sampled from sympatric and allopatric populations.SimulatedGenotypesGenotypes, simulated in HYBRIDLAB, at 12 diagnostics microsatellite loci for six genotypic classes of Ammodramus sparrows: pure caudacutus, backcrossed caudacutus, F1, F2, backcrossed nelsoni, and pure nelsoni.Assembled N50 contigs Ammodramus caudacutusFasta file of assembled contigs that were > the N50contig size of assembled Ammodramus caudacutus reads from Illumina sequencing.SALS-2-29-2012-assembly.fastaAssembled N50 contigs Ammodramus nelsoniFasta file of assembled contigs > than the N50 contig length for Illumina sequencing reads of Ammodramus nelsoni.Nelson-3-26-2012-assembly.fast