Soil biota is decisive for overyielding in intercropping under low phosphorus conditions

Cereal/legume intercropping typically increase crop yield, however, the mechanisms through which soil microbes mediate overyielding in intercropping under different nutrient availability remain elusive. Here we examined the effect of soil biota and phosphate (P) availability on intercropping advantages in wheat (Triticum aestivum) /faba bean (Vicia faba) systems using a pot experiment consisting of soil sterilization treatment and different P supply levels. A complementary experiment on the contribution of different microbial groups was also included using the removal method. Intercropping advantage was observed only in the presence of microbes and was mainly associated with increase of wheat biomass. Overyielding effect was stronger under low-P than high-P conditions, and was negatively correlated with P availability and the effect of soil biota in the monoculture. The effect was likely related to the enlarged active P pools and modified microbial communities associated with the alteration of rhizosphere traits (protons and carboxylates) by the neighboring faba bean. Similarly, simplification of soil microbes significantly increased the growth and P uptake of wheat plants while faba bean growth was less affected. Synthesis and applications. Our research provides compelling evidence that soil microbiome is important in driving intercropping overyielding by regulating interspecific interactions under nutrient limited conditions. These findings have important implications for crop species choice in designing and managing intercropping systems where crop functional traits, soil microbiome and nutrient management should be integrated in pursuit of sustainable agriculture.Funding provided by: National Natural Science Foundation of ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809Award Number: 31872182Funding provided by: National Key Research and Development Program of ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100012166Award Number: 2017YFD0200200Funding provided by: Applied Basic Research Project of Shanxi Province, ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100019447Award Number: 201901D111306Funding provided by: National Natural Science Foundation of ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809Award Number: 31560587Funding provided by: National Natural Science Foundation of ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809Award Number: 3156058