COMPARATIVE STUDY ON THE PROTECTIVE EFFICACY OF BETA-CAROTENE AND RESVERATROL (3 4 5 TRI_HYDROXYSTILLBENE) ON METHOTREXATE INDUCED HEPATOTOXICITY IN EXPERIMENTAL ANIMALS

Background: Methotrexate is an antimetabolite and antifolate drug. It is used in the therapy of leukemia, lymphoma and several solid tumors. . Beta-carotene can be stored in body and converted to retinol as needed, thus making it a form of vitamin A for humans and other animals. Resveratrol gets extensively metabolized in the body. Liver and Gut are the major sites of metabolism. Aim: The Aim of the study is to compare and evaluate the protective efficacy of Beta-Carotene on Methotrexate induced hepatic damage in rat liver and to compare and evaluate the protective efficacy of Resveratrol on Methotrexate induced hepatic damage in rat liver. Study Design: This is an experimental study. The study was conducted on 48 healthy Wister Albino rats obtained from animal house – BMSI JPMC, for 25 days from 13 August, 2018 to 05 September, 2018. Material And Methods: For present study, forty eight (n = 48) Wister Albino rats, either sex, weighing 210–310 Gm. were utilized. Rats were divided into six (6) groups randomly, each group including eight (n = 8) animals, grouped as Control (Group I), B-carotene only (Group II), Resveratrol only (Group III), MTX only (Group IV), B-carotene + MTX (group V) and Resveratrol + MTX (group VI). Results: (SPSS 17.0) was used for statistical analysis. The results were compared with paired t-test with p< 0.05 considered as statically significant. All results were expressed as means ± standard error (SEM). The mean value of initial body weight in control group I was 239.33±0.882 Gm and final body weight was 248.50±0.764 Gm. Conclusion: Based on present study it is concluded that methotrexate severely causes hepatic damage in rats, which can be protected by β-carotene and Resveratrol. Keywords: Methotrexate, Beta-carotene, Resveratrol