Data from: Differences in flowering time maintain species boundaries in a continental radiation of Viburnum

Premise of the study: We take an integrative approach in assessing how introgression and Pleistocene climate fluctuations have shaped the diversification of the Viburnum lentago clade, a group of five inter-fertile species with broad areas of sympatry. We specifically tested whether flowering time plays a role in maintaining species isolation. Methods: RAD-seq data for 103 individuals were used to infer the species relationships and the genetic structure within each species. Flowering times were compared among species based on historical flowering dates documented in herbarium specimens. Key Results: Within each species we found a strong relationship between flowering date and latitude, such that southern populations flower earlier than northern ones. In areas of sympatry, the species flower in sequence rather than simultaneously, with flowering dates offset by at least nine days for all species pairs. In two cases it appears that the offset in flowering times is an incidental consequence of adaptation to differing climates, but in the recently diverged sister species V. prunifolium and V. rufidulum, we find evidence that reinforcement led to reproductive character displacement. Long-term trends suggest that the two northern-most species are flowering earlier in response to recent climate change. Conclusions: We argue that speciation in the Lentago clade has primarily occurred through ecological divergence of allopatric populations, but differences in flowering time were essential to maintain separation of incipient species when they came into secondary contact. This combination of factors may underlie diversification in many other plant clades.lentago_collections_tableTable with individuals collected for this study. The state and county are listed for each individualPhenology_dataTable of phenology data collected from herbarium specimens. This file contains information on ther Herbarium, species, collector, collection number, locality, date, and phenological stage. In this file the phenology is scored: 0 = leaves, small flower buds, 1 = flower buds with expanded inflorescences, 2 = flowering, 3 = past flowering, small fruits visible, 4 = young fruits, 5 = mature fruits, 6 = fruits fallen off, 7 = no leaves or fruits (winter), NA = infertile.core_lentago-c88-d6-min24_outfilesAll ipyrad outfiles for the entire V. lentago clade + outgroups with all loci shared across at least 24 individuals.core_lentago-c88-d6-min49_outfilesAll ipyrad outfiles for the entire V. lentago clade + outgroups with all loci shared across at least 49 individuals.core_lentago-c88-d6-min73_outfilesAll ipyrad outfiles for the entire V. lentago clade + outgroups with all loci shared across at least 73 individuals.core_lentago_combined_floral_trait_dataMorphological trait data for the core Lentago species.cleaned_occurrences_lentago_cladeTable of occurrence information compiled from multiple sources and cleaned according to the processes described in our manuscript.Funding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: IOS-1256706, IOS-1257262, DGE-1122492, 150118