Data from: An unexpected clade of South American ground beetles (Coleoptera: Carabidae: Bembidion)

Phylogenetic relationships of the Antiperyphanes Complex of the genus Bembidion are inferred using DNA sequences from seven genes (two nuclear ribosomal, four nuclear protein coding, and one mitochondrial protein coding). Redefined subgenera within the complex are each well-supported as monophyletic. Most striking was the discovery that a small set of morphologically and ecologically heterogeneous species formed a clade, here called subgenus Nothonepha. This unexpected result was corroborated by the discovery of deep pits in the lateral body wall (in the mesepisternum) of all Nothonepha, a trait unique within Bembidion. These pits are filled with a waxy substance in ethanol-preserved specimens. In one newly discovered species (Bembidion tetrapholeon sp. n., described here), these pits are so deep that their projections into the body cavity from the two sides touch each other internally. These structures in Bembidion (Nothonepha) are compared to very similar mesepisternal pits which have convergently evolved in two other groups of carabid beetles. The function of these thoracic pits is unknown. Most members of subgenus Nothonepha have in addition similar but smaller pits in the abdomen. A revised classification is proposed for the Antiperyphanes Complex.DNA matrices and maximum likelihood treesThis is a NEXUS file containing the aligned DNA matrices, the optimal likelihood trees, and all of the original boostrap trees. If opened in Mesquite (version 2.75 or later), you will be able to see the majority consensus trees of the bootstrap runs and other details.PittedClade.MatricesAndTrees.nexBembidion tetrapholeon DNA variationThis is a NEXUS file containing the aligned data matrices for all the sequences gathered for specimens of Bembidion tetrapholeonBembidion tetrapholeon variation.nexMaximum Likelihood TreesThis file shows images of the maximum likelihood trees for the concatenated, 7-gene matrices as well as each individual gene. Each figure is labeled to indicate the nature of that data analyzed for that tree.MLTrees.pdfMaximum Likelihood Bootstrap treesThis file shows images of the majority rule consensus trees from maximum likelihood bootstrap analyses for the concatenated, 7-gene matrices as well as each individual gene. Each figure is labeled to indicate the nature of that data analyzed for that tree. Numbers on each branch are the frequencies of that clade in the bootstrap replicates, expressed as a percentage.MLBootstrapTrees.pd