Data from: Organization of biogeochemical nitrogen pathways with switch-like adjustment in fluctuating soil redox conditions

Nitrogen is cycled throughout ecosystems by a suite of biogeochemical processes. The high complexity of the nitrogen cycle resides in an intricate interplay between reversible biochemical pathways alternatively and specifically activated in response to diverse environmental cues. Despite aggressive research, how the fundamental nitrogen biochemical processes are assembled and maintained in fluctuating soil redox conditions remains elusive. Here, we address this question using a kinetic modelling approach coupled with dynamical systems theory and microbial genomics. We show that alternative biochemical pathways play a key role in keeping nitrogen conversion and conservation properties invariant in fluctuating environments. Our results indicate that the biochemical network holds inherent adaptive capacity to stabilize ammonium and nitrate availability, and that the bistability in the formation of ammonium is linked to the transient upregulation of the amo-hao mediated nitrification pathway. The bistability is maintained by a pair of complementary subsystems acting as either source or sink type systems in response to soil redox fluctuations. It is further shown how elevated anthropogenic pressure has the potential to break down the stability of the system, altering substantially ammonium and nitrate availability in the soil, with dramatic effects on biodiversity.Nitrogen_Biogeochemical_Network_Sanjay_Lamba_Chakraborty_ESMThis Auxiliary Material includes five supplementary tables (Table S1-S5), fifteen supplementary figures (Figs. S1-S15) and ten supplementary texts(Text S1-S10). Detailed methods and mathematical techniques are described in the supplementary texts; all the simulated model results in the form of tables and figures are presented in the auxiliary figures and tables. This material presents how the nitrogen biochemical system runs and is regulated over time and how it is responded to external ammonium supply