NEUROPROTECTIVE ACTIVITY OF HESPERIDIN AGAINST MPTP (1-METHYL-4-PHENYL-1, 2, 3, 6-TETRAHYDROPYRIDINE) INDUCED NEUROTOXICITY: IN VITRO APPROACHES

Aim: In the present study, we examined the neuroprotective activity of hesperidin on the accumulation of neuronal oxidative stress induced by MPTP by in vitro. Method: After decapitation, healthy rat brain was removed rapidly from the skull and rinsed with cold artificial cerebrospinal fluid (ACSF) which has been equilibrated with 95% O2/5 % CO2 gas mixture. Group I brain slices was incubated in ACSF serve as normal, Group II brain Slices was incubated in ACSF and DMSO (10%) serve as disease, Group III brain was incubated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (1 ng/ml) Group IV brain was incubated in MPTP with Bromocriptine (10 µg/ml) as a standard, Group V brain was incubated in MPTP with hesperidin (10 µg/ml) Group VI brain was incubated in MPTP with hesperidin (20 µg/ml). After 1hr of incubation brain slices were homogenized in PBS buffer, pH 7.4 and supernatant subjected estimation of for protein content, lipid peroxidase and reduced glutathione. Results and conclusion: MPTP incubated brain LPO* activity was significantly (p<0.001***) increased and GSH activity decreased (p<0.001***) compared to normal group. Hesperidin incubated group brain LPO* activity was significantly (p<0.05*) decreased and GSH activity was increased (p