Both diversity and functional composition affect productivity and water use efficiency in experimental temperate grasslands

Many experiments have shown that biodiversity promotes ecosystem functioning and stability and that this relationship varies with resource availability. However, we still have a poor understanding of the underlying physiological and ecological mechanisms driving diversity effects and how they may interact with soil nutrient availability. We collected data in a grassland experiment factorially manipulating fertilization, species richness, functional composition (slow-growing vs. fast-growing species), and functional diversity in resource economic traits. We measured aboveground productivity, nitrogen (N) uptake, photosynthesis, and water use efficiency by combining a 15N labelling approach with productivity, gas exchange, and stable isotope measurements in three years differing in rainfall. We found that sown species richness increased aboveground productivity, N uptake and photosynthesis, suggesting that species richness is the most important driver of ecosystem productivity and nutrient cycling. Similarly, photosynthesis was affected by functional composition but not by functional diversity. Water use efficiency was reduced by sown species richness for communities dominated by slow growing species but not for communities dominated by fast growing species. Fertilization increased productivity, N uptake and water use efficiency. The positive effects of high species richness on ecosystem functions were independent of fertility levels. Synthesis. Our results provide evidence that high species richness in temperate grasslands could enhance productivity and reduce the negative impacts of drought events. Multiple factors and community characteristics are important in driving enhanced ecosystem functioning in biodiverse grasslands and seem to affect functioning and stability through different mechanisms.Funding provided by: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001711Award Number: PZ00P2_179978,PZ00P3_174068The data collection and processing is described in full in the manuscript