Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

ABSTRACT: Bintaro (Cerbera odollam Gaertn). Plants belonging to the Apocynaceae family have properties such as antifungal, insecticide, antioxidant and antitumor. Bintaro fruit contains alkaloids, flavonoids, terpenoids, tannins and saponins. Bintaro plants are poisonous because they contain alkaloid group compounds which are toxic. This study aims to obtain data and information regarding the toxicity ratio of bintaro rind and bintaro fruit (Cerbera odollam Gaertn.) to Artemia salina Leach larvae by determining the value of Lethal Concentration 50 (LC50). This type of research was experimental with quantitative methods, using Artemia salina Leach larvae. Each extract was divided into 4 concentration groups. In the ethanol extract of bintaro fruit peel, namely at concentrations of 500, 1000, 1500 and 2500 ppm. Meanwhile, bintaro fruit extract at concentrations of 1000, 1500, 2000 and 2500 ppm, each of which was carried out 3 replications with 10 larvae. Data analysis using probit analysis showed that the ethanol extract of bintaro fruit peel (Cerbera odollam Gaertn.) had LC50 = 701.455 μg/mL which was categorized as toxic. Meanwhile, the ethanol extract of bintaro fruit (Cerbera odollam Gaertn.) has LC50 = 1199.5 μg/mL which is categorized as nontoxic