Discordant population structure among rhizobium divided genomes and their legume hosts

Symbiosis often occurs between partners with distinct life history characteristics and dispersal mechanisms. Many bacterial symbionts have genomes comprised of multiple replicons with distinct rates of evolution and horizontal transmission. Such differences might drive differences in population structure between hosts and symbionts and among the elements of the divided genomes of bacterial symbionts. These differences might, in turn, shape the evolution of symbiotic interactions and bacterial evolution. Here we use whole-genome resequencing of a hierarchically-structured sample of 191 strains of Sinorhizobium meliloti collected from 21 locations in southern Europe to characterize the population structures of this bacterial symbiont and its host plant Medicago truncatula. Sinorhizobium meliloti genomes showed high local (within-site) variation and little isolation by distance. This was particularly true for the two symbiosis elements pSymA and pSymB, which have population structures that are similar to each other, but distinct from both the bacterial chromosome and the host plant. The differences in population structure may result from among-replicon differences in the extent of horizontal gene transfer, although given limited recombination of the chromosome, different levels of purifying or positive selection may also contribute to among-replicon differences. Discordant population structure between hosts and symbionts indicates that geographically and genetically distinct host populations in different parts of the range might interact with genetically similar symbionts, potentially minimizing local specialization.Funding provided by: NSFCrossref Funder Registry ID: http://dx.doi.org/10.13039/100016620Award Number: PGRP-1856744Funding provided by: NSFCrossref Funder Registry ID: http://dx.doi.org/10.13039/100016620Award Number: IOS-1401864Funding provided by: NSFCrossref Funder Registry ID: http://dx.doi.org/10.13039/100016620Award Number: NSF IOS-1645875Funding provided by: JGICrossref Funder Registry ID: http://dx.doi.org/10.13039/100017812Award Number: CSP-503446Funding provided by: Carl R. Woese Institute for Genomic BiologyCrossref Funder Registry ID: http://dx.doi.org/10.13039/100017185Award Number:Here we use whole-genome resequencing of a hierarchically-structured sample of 191 strains of Sinorhizobium meliloti collected from 21 locations in southern Europe to characterize the population structures of this bacterial symbiont and its host plant Medicago truncatula. We extracted DNA from cultures of S. meliloti grown in liquid TY media using Qiagen DNeasy (Hilden, Germany) and sent samples to the DOE Joint Genome Institute (JGI) for sequencing (Berkeley, CA, USA). JGI prepared a paired end library for each strain, and sequenced samples on an Illumina HiSeq-2500 1TB platform (101nt read length; Illumina, Inc., San Diego, CA, USA). Of the 199 strains submitted to JGI, we received high-quality whole genome sequences for 166. We re-grew the remaining 33 strains from frozen cultures (as above), and extracted DNA using the Zymo Quick-DNA kit for Fungi or Bacteria (Irvine, CA, USA). These samples were sequenced (2 X 150 or paired end 150 nt read length) on the Novaseq 6000 platform (Illumina, Inc, San Diego, CA, USA) by the Roy J. Carver biotechnology center at the University of Illinois at Urbana-Champaign (USA). We successfully recovered quality S. meliloti genome sequences from 25 of these 33 isolates, for a total of 191 S. meliloti strains analyzed in this study