RP-HPLC Method Developed for the Estimation of Etodolac and Thiocolchicoside in Bulk and Combined Dosage Forms

A new, simple, specific, sensitive, rapid, accurate and precise RP HPLC method was developed for the estimation of Etodolac ETO and Thiocolchicoside THIO in bulk and combined pharmaceutical dosage forms. Etodolac ETO and Thiocolchicoside THIO was chromatographed on a Symmetry ODS C18 4.6mm—250mm, 5µm Column in a mobile phase consisting of Acetonitrile Methanol Acetate Buffer 25 20 55 v v . The mobile phase was pumped at a flow rate of 1.0 ml min with detection at 238 nm. The detector response was linear in the concentration of 200 600µg ml 20 60µg ml for Etodolac and Thiocolchicoside respectively. The intra and inter day variation was found to be less than 2 . The mean recovery of the drug from the solution was 99.98 . The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The RSD results for precision and intermediate precision found less than 2.0 . The LOD and LOQ were found to be 0.86 and 1.18 µg ml for Etodolac and 2.4 and 3.54µg ml for Thiocolchicoside respectively. The proposed method is simple, fast, accurate, precise and reproducible hence it can be applied for routine quality control analysis of Etodolac ETO and Thiocolchicoside THIO in bulk and combined pharmaceutical formulations. Prapulla Putta "RP-HPLC Method Developed for the Estimation of Etodolac and Thiocolchicoside in Bulk and Combined Dosage Forms" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29703.pd