ITS-Region Analysis for Medicinal Plants Identification

The ITS (Internal Transcribed Spacer) region in nuclear ribosomal DNA can be used not only for species identification but also for taxonomic clarification. The advantage for using this region is that it is a small sequence 600-1000bp in length (vary from species to species) and can be used even from a less amount of sample. The other advantage is that it is present in multiple copies so that after PCR (Polymerase Chain Reaction) produce sufficient concentration for further downstream processing together with purification, Restriction digestion, RAPD-RFLP analysis, sequencing, etc. Therefore this region sequencing can be used for authentic identification of medicinal plants. Further restriction analysis will be helpful in solving the ambiguity. This paper deals with the use of bioinformatics tool for restriction analysis of ITS-region sequencing data