Trends in the indicators of lipid peroxidation and antioxidant protection of rat blood serum and heart under acute experimental lung injury

Liuta O. O. Trends in the indicators of lipid peroxidation and antioxidant protection of rat blood serum and heart under acute experimental lung injury. Journal of Education, Health and Sport. 2018;8(1):320-329. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1341905 http://ojs.ukw.edu.pl/index.php/johs/article/view/5777 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 23.01.2018. Revised: 26.01.2018. Accepted: 31.01.2018. UDC 616.12-091.8-02:616.24-001-036.11-085]-092.9 TRENDS IN THE INDICATORS OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION OF RAT BLOOD SERUM AND HEART UNDER ACUTE EXPERIMENTAL LUNG INJURY O. O. Liuta SHEI I.Ya. Horbachevsky Ternopil State Medical University, Ministry of Public Health, Ukraine Abstract Dynamically, acute experimental lung injury (ALI), caused by intratracheal injection of chloride acid, revealed one-direction disorder of free radical oxidation processes in the blood serum and heart homogenate, the intensity of the processes increasing within 12 and 24 experimental hour. However, 48 hours after ALI simulation the growing concentration of TBA-active products was found in the blood serum only, while in the heart homogenate the index was found to be decreasing. In 72 hours, the blood serum revealed insignificant decrease in TBA-active products’ concentration. However, the index was about 3 times the control value. In the heart homogenate the index was found to decrease 72 hours after experiment start and was reaching the level of the control group. Owing to ALI simulation, the catalase activity in the blood serum exceeds the control level throughout the experiment, peak values found in 24 hours, whereas in the heart homogenate the index was found to be gradually decreasing till the 48th hour, remaining significantly lower as compared to the control level. Nevertheless, in 72 hours the catalase activity in the heart homogenate grows and exceeds the control level notably. Analysis of the antioxidant-pro-oxidant balance that was evaluated in the blood serum and heart homogenate by the API value revealed significant growth of the index in the blood serum within 12 and 24 hours as compared to the controls. However, by 72 hour the index was found to be decreasing reliably below the control level. Meanwhile, in the heart homogenate the API value is below the control level throughout 48 hours since ALI simulation, growing rapidly further and exceeding the control level notably in 72 hours. Key words: acute lung injury, lipid peroxidation, catalase, heart