pH Dependency of 'Hydration' Water and Iron(III) Complex Formation in Serum Albumin Solution

7705 Wabon Street, Au•tin, Texas, 78757-1539, U.S.A. Manuscript received 28 August 1995, revised 9 August 1996, accepted 18 November 1996 Spin-lattice (T1)- and spin-spin (T2) relaxation times of samples of experimental solutions of BSA have been determined in the pH range 7 2. While the reported structural changes arc indicated by the increases in T1s, the consequent diminutions of 'hydration' water, associated with the dissolved BSA molecules, are rationalised with the increases of T2s. This gradual loss of individual 'hydration' layers of those BSA molecules leads to the formation of 'molten globule' at pH