Data from: Heritable variation in an extended phenotype: the case of a parasitoid manipulated by a virus

In host-symbiont interactions, the genes of both host and symbiont can influence phenotypic traits. In the context of a conflict of interest, fitness-related traits are subjected to opposing selective pressures in the genomes of the partners. In the Drosophila parasitoid Leptopilina boulardi, females usually avoid laying eggs into already parasitized larvae. However, when infected by the virus LbFV, they readily lay additional eggs into parasitized larvae. Inducing superparasitism allows the virus to colonize uninfected parasitoid lineages, but is usually mal-adaptive for the parasitoid. We tested for the presence of resistance genes to this behavioural manipulation in the parasitoid genotype by sampling 30 lines from 5 populations with contrasting viral prevalence, after infecting them with a reference viral isolate. No geographical differentiation was observed although some genotypes underwent less manipulation than others and these differences were heritable across generations. The viral titre was not correlated to these differences, although fecundity differed between extreme lines.introgressionTable of superparasitism measurements in parasitoid females from the different generation of introgression. The first row correspond to designation of the different variables. "generation" indicates the generation of introgression. "Population" stands for the population of origin of the introgressed genotypes. "Line" is the name of the different introgressed genotypes. "female" gives the number of each tested female. "l1-l3" stands for the three dissected Drosophila larvae per parasitoid female. "Parasitism" is the mean number of parasitoid eggs per Drosophila larvae. "superparasitism" is the mean number of parasitoid eggs per parasitized drosophila larvae.isofemaleTable of superparasitism measurements of parasitoid females in isofemale lines. The first row corresponds to designation of the different variables. "Population" stands for the population of origin of isofemale lines. "Line" is the name of the different isofemale lines. "female" gives the number of each tested female. "l1-l5" stands for the five dissected Drosophila larvae per parasitoid female. "Parasitism" is the mean number of parasitoid eggs per Drosophila larvae. "superparasitism" is the mean number of parasitoid eggs per parasitized drosophila larvae. "infection" indicates the viral infection status of parasitoid females. "prevalence" indicates the viral prevalence in the populations of origin.expGQTable of superparasitism measurements in parasitoid females from the crossing experiment between "extreme" lines. The first row corresponds to designations of the different variables. "line" is the name of the different cross combinations. "mother" indicates the mother of tested females in each cross combination. "female" gives the number of each tested female. "l1-l3" stands for the three dissected Drosophila larvae per parasitoid female. "superparasitism" is the mean number of parasitoid eggs per parasitized drosophila larvae.fecundityTable of fecundity and tibia length measurements in parasitoid females from the extreme lines Av12 and Av3. The first row corresponds to designations of the different variables. "line" is the name of the extreme lines. "mother" indicates the mother of dissected females in each line. "female" gives the number of each dissected female. "fecundity" is the number of eggs per female. "tibia" is the tibia length of dissected females in µm.PCRQBC2Table of the viral titer in the four extreme lines Av3, Av8, Go16 and Sf12 at generation BC2. The first row corresponds to designations of the different variables. "line" is the name of the extreme lines. "virus" indicates the gene copy number for the viral molecular marker. "insect" indicates the gene copy number for the insect molecular marker. "ratio" indicates the ratio between the viral and the insect gene copy numbers