Features of neuro-endocrine and immune reactions to various water-salt loads in female rats

Gozhenko Olena A, Zavidnyuk Yuriy V, Korda Mykhaylo M, Mysula Igor R, Klishch Ivan M, Zukow Walery, Popovych Igor L. FEATURES OF NEURO-ENDOCRINE AND IMMUNE REACTIONS TO VARIOUS WATER-SALT LOADS IN FEMALE RATS. Journal of Education, Health and Sport. 2018;8(9):11-31. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1323491 http://ojs.ukw.edu.pl/index.php/johs/article/view/5729 https://pbn.nauka.gov.pl/sedno-webapp/works/872545 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part b item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eissn 2391-8306 7 © The Author(s) 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 20.06.2018. Revised: 28.06.2018. Accepted: 30.07.2018. FEATURES OF NEURO-ENDOCRINE AND IMMUNE REACTIONS TO VARIOUS WATER-SALT LOADS IN FEMALE RATS Olena A Gozhenko1, Yuriy V Zavidnyuk1,2, Mykhaylo M Korda2, Igor R Mysula2, Ivan M Klishch2, Walery Zukow3, Igor L Popovych4 1Ukrainian Scientific Research Institute of Transport Medicine, Odesa, Ukraine, olena.gozhenko@gmail.com 2IY Horbachevs’kyi State Medical University, Ternopil’, Ukraine, zavidnyukyv@tdmu.edu.ua 3Nicolaus Copernicus University, Torun, Poland, w.zukow@wp.pl 4OO Bohomolets’ Institute of Physiology, Kyiv, Ukraine, i.popovych@biph.kiev.ua Abstract Background. Previously, we have shown that the weekly load of rats with water-salt solutions of different chemical compositions causes both general and specific reactions of the parameters of metabolism. The purpose of this study is to identify under these conditions specific neuroendocrine and immune responses. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats some neuroendocrine and immune parameters were registered. Results. The method of discriminant analysis revealed 29 parameters of the neuroendocrine-immune complex (10 of them reflect the neuroendocrine regulation, 4 thymus mass and thymocytogram elements, 5 elements of splenocytogram, 10 elements of immunocytogram and leukocytogram of blood and parameters of phagocytosis), according to which the reaction on various water-salt loads are identified with an accuracy of 98.3%. Conclusion. The peculiarities of the reactions of the parameters of the neuroendocrine-immune complex are due to the content of water in sulfate, bicarbonate and magnesium, as well as organic carbon and nitrogen. Key words. Water-salt loads, neuroendocrine and immune parameters, female rats.Gozhenko Olena A, Zavidnyuk Yuriy V, Korda Mykhaylo M, Mysula Igor R, Klishch Ivan M, Zukow Walery, Popovych Igor L. FEATURES OF NEURO-ENDOCRINE AND IMMUNE REACTIONS TO VARIOUS WATER-SALT LOADS IN FEMALE RATS. Journal of Education, Health and Sport. 2018;8(9):11-31. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1323491 http://ojs.ukw.edu.pl/index.php/johs/article/view/5729 https://pbn.nauka.gov.pl/sedno-webapp/works/87254