YTK Primer Design

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).

Breunig, C.
Durovic, T.
Neuner, A.M.
Baumann, V.
Wiesbeck, M.F.
Köferle, A.
Götz, M.
Ninkovic, J.
Stricker, S.H.

January 2018

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and e...

Primer3–new capabilities and interfaces

Andreas Untergasser
Ioana Cutcutache
Triinu Koressaar
Jian Ye
Brant C. Faircloth
Maido Remm
Steven G. Rozen

January 2012

Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

McCarty, NS
Graham, AE
Studená, L
Ledesma-Amaro, R

February 2020

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...

YTK Primer Design

Skrekas Christos
Maximilian Otto
Michael Gossing
Johan Gustafsson
Jens Nielsen
Verena Siewers
Florian David

June 2021

Two apps included in the publication Skrekas, C., Otto, M., Gossing, M., Gustafsson, J., Nielsen, J....

Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries

Otto, Maximilian
Skrekas, Christos
Gossing, Michael
Gustafsson, Johan
Siewers, Verena
David, Florian

January 2021

Standardisation of genetic parts has become a topic of increasing interest over the last decades. Th...

CRISPR/Cas9: A molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

Mans, R. (author)
Van Rossum, H.M. (author)
Wijsman, M. (author)
Backx, A. (author)
Kuijpers, N.G.A. (author)
van den Broek, M. (author)
Daran-Lapujade, P.A.S. (author)
Pronk, J.T. (author)
Van Maris, A.J.A. (author)
Daran, J.G. (author)

March 2015

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently been develo...

RESEARCH ARTICLE CRISPR MultiTargeter: A Web Tool to Find Common and Unique CRISPR Single Guide RNA Targets in a Set of Similar Sequences

Sergey V. Prykhozhij
Vinothkumar Rajan
Daniel Gaston
Jason N. Berman

August 2016

Genome engineering has been revolutionized by the discovery of clustered regularly inter-spaced pali...

Genome Screen Reports Primers-4-Yeast: a comprehensive web tool for planning primers for Saccharomyces cerevisiae

Ido Yofe
Maya Schuldiner
Correspondence To M. Schuldiner

January 2013

The budding yeast Saccharomyces cerevisiae is a key model organism of functional genomics, due to it...

A modular cloning toolkit for genome editing in plants

Hahn, F
Korolev, A
Sanjurjo Loures, L
Nekrasov, V

January 2020

Background CRISPR/Cas has recently become a widely used genome editing tool in various organisms, in...

A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

Novarina, Daniele
Koutsoumpa, Andriana
Milias-Argeitis, Andreas

June 2022

CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, an...

CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries.

Becker, Martin
Noll-Puchta, Heidi
Amend, Diana
Nolte, Florian
Fuchs, Christiane
Jeremias, Irmela
Braun, Christian J

January 2020

Becker M, Noll-Puchta H, Amend D, et al. CLUE: a bioinformatic and wet-lab pipeline for multiplexed ...

A Modular Cloning Toolkit Including CRISPRi for the Engineering of the Human Fungal Pathogen and Biotechnology Host Candida glabrata

Billerbeck, Sonja
Prins, Rianne C.
Marquardt, Malte

April 2023

The yeast Candida glabrata is an emerging, often drug-resistant opportunistic human pathogen that ca...

Development and application of the Landing Pad platform: A synthetic CRISPR/Cas9 platform for multi-copy gene integration in Saccharomyces cerevisiae

Bourgeois, Leanne

April 2018

To accelerate the construction of superior yeast strains producing high-value chemicals, we develope...

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Christopher T. Breunig (5150645)
Tamara Durovic (5150639)
Andrea M. Neuner (5150642)
Valentin Baumann (3481214)
Maximilian F. Wiesbeck (5150636)
Anna Köferle (3481229)
Magdalena Götz (32199)
Jovica Ninkovic (104237)
Stefan H. Stricker (180122)

April 2018

<div><p>Novel applications based on the bacterial CRISPR system make genetic, genomic, transcription...

Designing yeast cell factories to produce early-step Taxol® precursors: from CRISPR toolkit development to computer-aided design and optimisation of a CRISPR-based molecular diagnosis method

Malci, Koray

December 2022

CRISPR methods for yeast generally rely on pre-assembled DNAs and extra cloning steps to deliver g...

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).

Breunig, C.
Durovic, T.
Neuner, A.M.
Baumann, V.
Wiesbeck, M.F.
Köferle, A.
Götz, M.
Ninkovic, J.
Stricker, S.H.

January 2018

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and e...

Primer3–new capabilities and interfaces

Andreas Untergasser
Ioana Cutcutache
Triinu Koressaar
Jian Ye
Brant C. Faircloth
Maido Remm
Steven G. Rozen

January 2012

Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

McCarty, NS
Graham, AE
Studená, L
Ledesma-Amaro, R

February 2020

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...

YTK Primer Design

Skrekas Christos
Maximilian Otto
Michael Gossing
Johan Gustafsson
Jens Nielsen
Verena Siewers
Florian David

June 2021

Two apps included in the publication Skrekas, C., Otto, M., Gossing, M., Gustafsson, J., Nielsen, J....

Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries

Otto, Maximilian
Skrekas, Christos
Gossing, Michael
Gustafsson, Johan
Siewers, Verena
David, Florian

January 2021

Standardisation of genetic parts has become a topic of increasing interest over the last decades. Th...

CRISPR/Cas9: A molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

Mans, R. (author)
Van Rossum, H.M. (author)
Wijsman, M. (author)
Backx, A. (author)
Kuijpers, N.G.A. (author)
van den Broek, M. (author)
Daran-Lapujade, P.A.S. (author)
Pronk, J.T. (author)
Van Maris, A.J.A. (author)
Daran, J.G. (author)

March 2015

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently been develo...

RESEARCH ARTICLE CRISPR MultiTargeter: A Web Tool to Find Common and Unique CRISPR Single Guide RNA Targets in a Set of Similar Sequences

Sergey V. Prykhozhij
Vinothkumar Rajan
Daniel Gaston
Jason N. Berman

August 2016

Genome engineering has been revolutionized by the discovery of clustered regularly inter-spaced pali...

Genome Screen Reports Primers-4-Yeast: a comprehensive web tool for planning primers for Saccharomyces cerevisiae

Ido Yofe
Maya Schuldiner
Correspondence To M. Schuldiner

January 2013

The budding yeast Saccharomyces cerevisiae is a key model organism of functional genomics, due to it...

A modular cloning toolkit for genome editing in plants

Hahn, F
Korolev, A
Sanjurjo Loures, L
Nekrasov, V

January 2020

Background CRISPR/Cas has recently become a widely used genome editing tool in various organisms, in...

A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

Novarina, Daniele
Koutsoumpa, Andriana
Milias-Argeitis, Andreas

June 2022

CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, an...

CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries.

Becker, Martin
Noll-Puchta, Heidi
Amend, Diana
Nolte, Florian
Fuchs, Christiane
Jeremias, Irmela
Braun, Christian J

January 2020

Becker M, Noll-Puchta H, Amend D, et al. CLUE: a bioinformatic and wet-lab pipeline for multiplexed ...

A Modular Cloning Toolkit Including CRISPRi for the Engineering of the Human Fungal Pathogen and Biotechnology Host Candida glabrata

Billerbeck, Sonja
Prins, Rianne C.
Marquardt, Malte

April 2023

The yeast Candida glabrata is an emerging, often drug-resistant opportunistic human pathogen that ca...

Development and application of the Landing Pad platform: A synthetic CRISPR/Cas9 platform for multi-copy gene integration in Saccharomyces cerevisiae

Bourgeois, Leanne

April 2018

To accelerate the construction of superior yeast strains producing high-value chemicals, we develope...

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Christopher T. Breunig (5150645)
Tamara Durovic (5150639)
Andrea M. Neuner (5150642)
Valentin Baumann (3481214)
Maximilian F. Wiesbeck (5150636)
Anna Köferle (3481229)
Magdalena Götz (32199)
Jovica Ninkovic (104237)
Stefan H. Stricker (180122)

April 2018

<div><p>Novel applications based on the bacterial CRISPR system make genetic, genomic, transcription...

Designing yeast cell factories to produce early-step Taxol® precursors: from CRISPR toolkit development to computer-aided design and optimisation of a CRISPR-based molecular diagnosis method

Malci, Koray

December 2022

CRISPR methods for yeast generally rely on pre-assembled DNAs and extra cloning steps to deliver g...

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).

Breunig, C.
Durovic, T.
Neuner, A.M.
Baumann, V.
Wiesbeck, M.F.
Köferle, A.
Götz, M.
Ninkovic, J.
Stricker, S.H.

January 2018

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and e...

Primer3–new capabilities and interfaces

Andreas Untergasser
Ioana Cutcutache
Triinu Koressaar
Jian Ye
Brant C. Faircloth
Maido Remm
Steven G. Rozen

January 2012

Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

McCarty, NS
Graham, AE
Studená, L
Ledesma-Amaro, R

February 2020

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...

YTK Primer Design

Abstract

Extracted data

YTK Primer Design

Abstract

Extracted data

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