Data from: Loci controlling nitrate reductase activity in maize: ultraviolet-B signaling in aerial tissues increases nitrate reductase activity in leaf and root when responsive alleles are present

Environmental factors, such as ultraviolet-B (UV-B) irradiation, have the ability to affect pathways such as nitrogen metabolism. As fixed nitrogen is the keystone mineral nutrient that controls grain crop yield, any alteration in this cycle can be detrimental to plant productivity. Nitrate reductase enzyme activity is responsible for the reduction of nitrate to nitrite, and nitrate is the major form of nitrogen assimilated in plants. In maize (Zea mays L.) production, nitrate assimilation kinetics are important for both high- and low-input agricultural systems. Nitrate reductase protein activity is controlled by phosphatases and kinases. Nitrate reductase activity is responsive to environmental signals such as light–dark cycles and UV-B radiation, although the regulatory controls are not yet fully understood. We have determined the location of maize genetic factors that control nitrate reductase activity and the extent of contribution of each of these factors, both locally in the leaf tissue and via long-distance signaling loci that affect root nitrate reductase activity upon leaf UV irradiation. In the IBM94 recombinant inbred mapping population, the loci controlling regulation of nitrate reductase activity under UV-B map to different positions than the loci controlling nitrate reductase activity in unexposed plants.PPL_1406_sm_AppendixS1Construction of simulations, results of comparison of GLMC and composite interval mapping; details of GLM mixed model for QTL detection, and comparison of QTL detected with in pilot and full experiment and by GLMC and QTLNetwork composite interval mapping.Copy of PPL_1406_sm_TableS1Raw and adjusted trait data for each sample for pilot and full experiment.Copy of PPL_1406_sm_TableS2Expression-QTL genes under leaf nitrate reductase activity QTL. Markers within the nitrate reductase activity QTL region were used to select markers that had significant linkage to expression differences in genes that mapped to the same chromosomal bin. The MZ_IDs for the array oligonucleotides have only been mapped to bin in the B73 Refgen1 sequence, not to the exact genomic location, so some of these eQTL may not be within the nitrate reductase activity QTL region within that bin.Copy of PPL_1406_sm_TableS3Genes located in the same chromosomal region as nitrate reductase activity QTL regions. Flanking genetic markers were localized on the B73 sequence and all genes in the filtered gene set that had genomic locations between those co-ordinates were selected