Catalysis of MUF-triNAG by hen egg-white lysozyme (dataset formatted for analysis by interferENZY)

Main description This dataset depicts the catalysis of the fluorogenic substrate MUF-triNAG to the fluorescent substrate MUF by hen egg-white lysozyme, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in Phys. Chem. Chem. Phys., 2020, 22, 16143-16149 (https://doi.org/10.1039/D0CP02469D) and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis. Dataset organization Line 1: Tab-separated initial concentrations of substrate MUF-triNAG in micromolar (µM) concentration Line 2: Concentration of enzyme (0.25 µM) Line 3: Units of time Line 4: Units of concentration for substrate values and measurements Line 5: Dataset name Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1 Contact information: Maria Filipa Pinto (mfpinto@i3s.up.pt) Pedro M. Martins (pmartins@ibmc.up.pt) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: +351 226 074 900Acknowledgements: This work was financed by (i) the European Regional Development Fund (ERDF) through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia (FCT/MCTES) in the framework of grants POCI-01-0145-FEDER-031173, POCI-01-0145-FEDER-007274, POCI-01-0145-FEDER-031323 ("Institute for Research and Innovation in Health Sciences"), (ii) Base funding – UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE – funded by national funds through FCT/MCTES (PIDDAC), and by (iii) the ERDF through Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement in the framework of Project Norte-01-0145-FEDER-000008. MFP gratefully acknowledges PhD fellowship SFRH/BD/109324/2015 from FCT, Portugal