Benzocaine derivative determination via furan-2-carboxaldehyde reagent using differential pulse polarographic method

Benzocaine (BE) has been determined by the indirect differential pulse polarographic method (DPP). The suggested method is based on the synthesis of benzocaine via furan -2-carboxaldehyde reagent in presence of hydrochloric acid, then the diazotized at 0-5 oC with sodium nitrite to obtain a new benzocaine derivative. Also finding the values half-wave potential (E½) and electrochemical behavior using the dropping mercury electrode (DME). Optimum conditions affecting a quantitative analysis of benzocaine and its derivative such as solvent, pH, buffer solution and supporting electrolyte effect were explored. The benzocaine derivative showed a clear reduction peak at a potential of (-0.93 V) under optimal conditions. The standard calibration curve prepared for the benzocaine derivative at a concentration range of (5-100) µg.ml-1 gave a correlation coefficient of 0.9998, also limit of detection (LOD) and limit of quantitation (LOQ) of the benzocaine drug was 20.096 and 6.949 µg.ml-1 respectively, where the average relative standard deviation RSD of the benzocaine derivative was 0.41 % with recovery 100.40% (n=5). Based on the observation, the developed method could be employed for the estimation of benzocaine in pharmaceutical formulations