THE EFFECTIVENESS OF STEM CELLS GRAFTING ON REGENERATION OF DENTAL PULP: A SYSTEMATIC REVIEW AND META-ANALYSIS

Background: Great advances in tissue engineering open a new biological path to regenerate the damaged pulpal tissue. The new approach of stem cell transplantation to regeneration of tissues had been used in many medical fields with promising results including dental therapy. Aim of this istoreviewthe efficacy of stem cell grafting in regeneration of dental pulp from available animal and human studies for a systematic and meta-analysisreview. Methodology: AComprehensive electronic search with time and language restrictions was conducted. Several known databases were included Ex: PubMed, The Cochrane Library, Web of Science from 2000 to 2020.We combined the search terms and limited the study to the English language. Depending on PRISMA checklist, we removed duplicates, articles were screened based on title, abstract, and full text. The search resulted in 325 hits which after removing duplicates, exclusion studies the number of studies became 10. Results:In this review, The total samples used in this review was 40 samples from which 13 human patients had been included (mean age of 26.8 years old), 12 dogs, 3 mice and 12 inbred male miniature pigs using 94 teeth and molar. Moreover, all the used stem cells for dentine regeneration were adult mesenchymal stem cells however, the source of harvesting these stem cell differs between studies including adipose tissue and pulp CD31-SP, DPSCS, PDLSCs, Gdf11 gene,bone morphogenetic protein-2 (BMP-2),MDPSCs and SCAP. Furthermore, all types showed promising results however, some types gave better results over other including superior of adipose tissue CD31 SP over bone marrow CD31 SP and pulp stem progenitor (CD 105) cells over adipose CD105 cell. Conclusion: It was observed that stem cell grafting shows promising results in the regeneration of dental pulp in both animal and human studies with no side effect or toxicity. Therefore, we recommend widening the application of these techniques in human trials because of its safety and efficacy. Choice of carriers or type of stem cells up to our review should depend on the expenses, as there are no significant differences between them in both safety of efficacy profiles