METHOD DEVELOPMENT AND VALIDATION OF PROPRANOLOL HCL BY UV SPECTROSCOPIC METHOD IN A BULK AND PHARMACEUTICAL DOSAGE FORM

A new simple, accurate, rapid, precise, reproducible and cost-effective spectrophotometric method for the quantitative estimation of Propranolol Hcl in bulk and pharmaceutical dosage form. The developed UV spectrophotometric method for the quantitative estimation of Propranolol Hcl is based on measurement of absorption at maximum wavelength 228 nm using Acetonitrile with buffer as a solvent. The stock solution of Propranolol Hcl was prepared, and subsequent suitable dilution was prepared in acetonitrile: buffer to obtained standard curve. The standard solution of Propranolol Hcl shows absorption maxima at 228 nm. The drug obeyed beer lambert’s law in the concentration range of 5 - 25 μg/ml with regression 0.999 at 228 nm. The overall % recovery was found to be 99.03% which reflects that the method was free from the interference of the impurities and other excipients used in the bulk and marketed dosage form. The low value of % RSD was indicative of accuracy and reproducibility of the method. The % RSD for inter-day and intra-day precision was found to be 0.173 and 0.17 respectively which is