Data from: Cascading reproductive isolation: plant phenology drives temporal isolation among populations of a host-specific herbivore

All organisms exist within a complex network of interacting species, thus evolutionary change may have reciprocal effects on multiple taxa. Here, we demonstrate "cascading reproductive isolation," whereby ecological differences that reduce gene flow between populations at one trophic level affect reproductive isolation (RI) among interacting species at the next trophic level. Using a combination of field, laboratory and common-garden studies and long-term herbaria records, we estimate and evaluate the relative contribution of temporal RI to overall prezygotic RI between populations of Belonocnema treatae, a specialist gall-forming wasp adapted to sister species of live oak (Quercus virginiana and Q. geminata). We link strong temporal RI between host-associated insect populations to differences between host plant budbreak phenology. Budbreak initiates flowering and the production of new leaves, which are an ephemeral resource critical to insect reproduction. As flowering time is implicated in RI between plant species, budbreak acts as a "multitrophic multi-effect trait," whereby differences in budbreak phenology contribute to RI in plants and insects. These sister oak species share a diverse community of host-specific gall-formers and insect natural enemies similarly dependent on ephemeral plant tissues. Thus, our results set the stage for testing for parallelism in a role of plant phenology in driving temporal cascading RI across multiple species and trophic levels.Herbaria recordsHerbaria records used in analysis of flowering time.Herbarium Records.xlsxBudbreak in NatureData used to estimate live oak budbreak phenologyInitial Budbreak Common GardenDate of first budbreak in sapling live oaks reared in a common gardenEmergence in NatureAdult B. treatae caught in traps used to estimate emergence phenology in natureEmergence in LaboratoryAdult B. treatae emerged from root galls in the laboratory used to estimate emergence phenologyAdult LongevityMeasurements of adult B. treatae longevit