Virulence gene expression of Pseudomonas aeruginosa isolated from children with nephrotic syndrome

Pseudomonas aeruginosa is Gram-negative, and considered as the most common opportunistic pathogens which lead to high rate of mortality and morbidity when subject suffered from compromised immune systems. Objectives: This study was to estimate the lasI and lasR genes expression of P. aeruginosa isolated from children with nephrotic syndrome. Methods: Nineteen isolates from urine samples of children with nephropathy syndrome are included in this study and confirmed by VITEK test as a Pseudomonas aeruginosa strains. Results and Conclusions: The result showed that the LasI and LasR genes showed a high level of gene expression in strains that grown with ceftriaxone than the strains that grown alone, but the gene LasI had a higher level of expression than the LasR gene, also a regression analysis were done to reveal the effect of LasI on the LasR gene which showed a positive effect which mean any increment in the gene expression level of LasI gene will combined with an increment in the LasR gene, (R= 0.899 and P= 0.001)