Design and Synthesis of Novel Chromeno Pyrimidine analogs of potential Antimicrobial properties

The aim of the study is to design and synthesize the new bioactive heterocyclic compounds. Pyrimidine is an important class of heterocyclic compound that are known to possess important pharmacological and antimicrobial properties. 2H-chromenes are also known to possess antimicrobial properties. Chromeno pyrimidines are compounds having both pyrimidines and 2H-chromenes in their structures. Hence, the synthesis of novel chromeno pyrimidine containing chloro, fluoro, trifluoromethyl groups have been carried out by multi-step organic synthesis, in order to study their interesting antimicrobial properties. The synthesized compounds were characterized by 1H NMR, 13C NMR, 19F NMR, FT-IR, GC-MS and elemental analysis. They were screened for their antibacterial activities against Gram-positive (Staphylococcus aureus, Bacillus subtilis), Gram negative (Escherichia coli, Pseudomonas aeruginosa) bacteria and anti-fungal activities against Aspergillus niger and Candida albicans by cup plate method. The fluoro and trifluro methyl derivatives showed the highest antimicrobial activity compared to the others. The trifluoro derivative (6c2) exhibited excellent results with the highest inhibition zone of 26mm in 100 µg/ mL against Bacillus subtilis and 23mm in 100 µg/ mL against Pseudomonas aeruginosa