Data from: Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays

Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.Contigs sequences analyzed in this study.This file with txt extension, contains the analyzed all contigs in this study.454_contigs.txtGenotypes, variants and other information from 143 SNPs analyzed143 SNP name, variants, dataset of the contig (source), annotation, GenBank accession number, external PCR primer sequences (Forward and Reverse), tailed extension primer (showing tails in lower case), extension primer direction and multiplexs are shown. Unfeasible SNPs are indicated in red colour.Genotypes_variants.xlsxInformation from samples 89 genotype ddRad loci identified.This file contains information from samples 89 genotyped .Information_samples.xlsxThis file contains the access information in NCBI of 39 samples used of to develop the SNPs.This file contains the access information in NCBI of 39 samples used to develop the SNPs.Table_ information_NCBI(SRA).doc