CRISPR/Cas9 knock-out sgRNA sequencing in human CaCo2 cells

The data was obtained in course of performing a CRISPR/Cas9 knock-out in human polarized epithelial cells (CaCo2). The screen aimed to identify genes relevant for proper epithelial polarization and correct apical transport of the apical transmembrane protein dipeptidyl-peptidase 4. It includes next-generation-sequencing data (raw sgRNA-read-counts) of two experimental (sorted) and corresponding control (ctrl) cell populations. It further contains sequence information on the sgRNAs as represented in the human GeCKOv2-CRISPR library and the corresponding targeted genes.Funding provided by: Austrian Science FundCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100002428Award Number: Funding provided by: Oesterreichische NationalbankCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100004061Award Number: Funding provided by: Österreichischen Akademie der WissenschaftenCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001822Award Number