COMPARATIVE STUDIES OF DIFFERENT DNA EXTRACTION METHODS FOR PCR – BASED ASSAY IN ARACHIS HYPOGAEA ( L. )

High quality DNA extractions are a prerequisite for genetic studies for a variety of plants including Arachis hypogea (L.) Nowadays, there are great number of plant DNA extraction method and commercially available extraction kit are also becoming more and more popular. It appears that different procedures work best for different plant groups. Thus in the genetic studies of A.hypogea, choosing CTAB method to choose becomes a concern. The DNA extracted by this method from (Control and Seaweed Liquid Fertilizer (SLF) treated fresh young leaf tissue of A.hypogea was analysed according to their cost and time, yield, purity, integrity and PCR (Polymerase Chain Reaction) based downstream analysis. The quality and quantity of isolated DNA was measured by Nano photometer. The absorption value A260/280 was calculated. Based on OD values the 10% and 25% of SLF treated plants showed pure and contaminant free DNAs when compared to control plants. Further the isolated genomic DNA was checked with 0.8% agarose gel electrophoresis stained with ethidium bromide to check the DNA quality. The gel was photographed under gel documentation system. In addition, the quantity and quality of the DNA extracted by this method were high enough to perform hundreds of PCR based reactions. Key words: DNA purification, Nano photometer, PCR, extractions