Simultaneous determination of Glipizide and Metformin hydrochloride in pharmaceutical preparation by HPLC

Department of Chemistry, University of Allahabad, Allahabad-211 002, India Manuscript received 10 October 2002, revised 4 June 2003, accepted 4 July 2003 A new HPLC method for the determination of Glipizide and Metformin hydrochloride in combination has been described. The method is based on reverse phase liquid chromatography using C-18 column and a suitable moblie phase. The detection is done at 225 nm. The flow rate is adjusted at 1.0 ml/min and linearity is established