Structure of C73G putidaredoxin from Pseudomonas putida

The structure of the C73G mutant of putidaredoxin (Pdx), the Fe2S2 ferredoxin that supplies electrons to cytochrome CYP101 (P450cam) for camphor oxidation, is reported at 1.9 Å resolution in a C2 crystal form. The structure was solved by single-wavelength iron anomalous diffraction, which yielded electron density above the 2σ level for over 97% of the non-H atoms in the protein. The final structure with R = 0.19 and Rfree = 0.21 has been deposited in the Protein Data Bank with accession code 1r7s. The C2 crystal contains three Pdx molecules in the asymmetric unit, giving three independent models of the protein that are very similar (r.m.s.d