Antibacterial Study of Silver, Copper, Gold, and Titanium Dioxide Nanoparticles Prepared by DC and RF Magnetron Sputtering

In this work, the antibacterial activity of nanomaterial’s copper, silver, gold and titanium dioxide sportily was investigated on both gram positive and negative bacteria. Nanoparticles of Cu, Ag, Au and TiO2 films were grown on glass substrates by dc and RF magnetron sputtering techniques. Nanoparticles films deposition were carried out at optimized argon pressure of 5.5×10-2mbar, sputtering plasma power of 30 Watt for Cu, Ag and Au samples and pressure of 1×10-3 mbar, plasma sputtering power of 100 W for TiO2 samples. Escherichia coli, Pseudomonas aeruginosa, and staphylococcus aurous were used to evaluate antibacterial activity. In vitro antibacterial analysis indicated that significantly reduced number of used Escherichia coli, Pseudomonas aeruginosa, and staphylococcus aurouswere detected on Agnanoparticles surface compared to an coated substrate surface. Both Cu and Au nanoparticles had inhibited some of pathogenic bacteria and observed over sample area. In the case of TiO2 films the abatement of bacteria, the antibacterial kinetics was observed to occur with the 120 hrs