SIGNIFICANCE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN SKIN UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA AND CORRECTION OF THEIR DISORDERS BY THIOTRIAZOLINE

Regeda-Furdychko M. M. SIGNIFICANCE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN SKIN UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA AND CORRECTION OF THEIR DISORDERS BY THIOTRIAZOLINE. Актуальні проблеми транспортної медицини / Actual problems of transport medicine / 2019;4(58):143-150. ISSN 1818-9385 DOI http://dx.doi.org/10.5281/zenodo.3590388 http://aptm.org.ua SIGNIFICANCE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN SKIN UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA AND CORRECTION OF THEIR DISORDERS BY THIOTRIAZOLINE Regeda-Furdychko M. M. Lviv Medical Institute The purpose of this study was to investigate the features of changes in the processes of lipoperoxidation and antioxidant protection in the skin during the formation of experimental contact dermatitis (ECD) and experimental pneumonia (EP) before and after the administration of the antioxidant thiotriazoline. Materials and methods. Researches were conducted on guinea pigs, divided into five groups: I — control, II — 4th day of experiment, III — 8th day of contact dermatitis development and experimental pneumonia, IV — 10th day and V — 18th day of model processes and VI group — guinea pigs with ECD and EP after treatment with thiotriazoline from 8 th to the 18th day of the experiment during 10 days. Early period included groups of animals on the 4th and 8th days of experiment. The late one — guinea pigs on the 8th and 18 th days of ECD and EP. Experimental contact dermatitis was simulated by method of Volkova V.A. (2010). EP was called by the method of Shlyapnikov V.N., Solodov T.L. (1998). Thiotriazoline was administered intramuscularly at a dose of 100 mg per 1 kg of weight daily from the 10 th to the 18 th day of the experiment. Condition of free radical lipid oxidization in the skin was determined on maintenance content of malonic dialdehyde by method of Corobeynikov E.G. (1989) and diene conjugates by method of Gavrylov V.G., Myshkorudna M. I. (1989). The degree of activity of antioxidant defence was estimated on maintenance enzymes — superoxidedismutase by method of R. Fried (1975), catalase by the method of B. Holmes, C. Masters (1970), glutationperoxidase method of Arkhipova O. G. (1988) and glutationreductase method of Moina V.M. (1986). Results and discussion.The paper deals with the growth of free radical oxidation products — diene conjugates and malondialdehyde in guinea pig skin with experimental contact dermatitis and experimental pneumonia. Reduced activity of antioxidant protection — superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase, especially expressed on the 18th day of the experiment. The data obtained indicate an increase in lipid peroxidation amid the depletion of compensatory reactions. The use of the thiotriazoline antioxidant has a corrective effect on altered indices of the pro- and antioxidant systems in guinea pig skin under conditions of experimental combined pathology. Key words: contact dermatitis, pneumonia, lipid peroxidation, antioxidant system, thiotriazoline