Data from: Spruce and beech as local determinants of forest fungal community structure in litter, humus and mineral soil

Beech forests reaches its native distribution limit in SE Norway, but is expected to expand substantially northwards due to climate warming. This may potentially result in a fundamental transformation of contemporary Northern European forests, with tentative effects on the associated belowground fungi. Fungal communities mediate vital ecosystem processes such as ecosystem productivity and carbon sequestration in boreal forests. To investigate how soil fungi is affected by the vegetation transition from spruce to beech forest, we sampled litter, humus and mineral soil in a forest landscape dominated by beech, spruce or a mixture of these. The fungal communities in the soil samples were analyzed by DNA metabarcoding of the rDNA ITS2 region. Although soil layers were the most important structuring gradient, we found clear differences in fungal species composition between spruce and beech plots. The differences in fungal community composition were most evident in the litter and least in the mineral soil. Decomposers, most notably Mycena, dominated the litter layer while various mycorrhizal fungi dominated the humus and mineral layers. Some ectomycorrhizal taxa, such as Cenoccocum and Russula, were more abundant in spruce forests. Differences in fungal community composition between forest types can potentially have large impacts on carbon sequestration rates.Representative sequences for all OTUsUnclustered representative sequences for all OTUsFF_ITS_NC_uclustref_repset.fnaFF7_ITS2mapBarcode and primer mapping fileFF8_ITS2mapBarcode and primer mapping fileFinal OTU/Sample data matrixOTU-table with taxonomic annotation for OTUs and information on sample treatmentsFinal OTU-Sample data matrix.xlsxRaw sequences (forward reads) first partFirst part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.001Raw sequences (forward reads) second partSecond part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.002Raw sequences (forward reads) third partThird part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.003Raw sequences (reverse reads) first partFirst part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.001Raw sequences (reverse reads) second partSecond part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.002Raw sequences (reverse reads) third partThird part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.003Raw sequences (reverse reads) fourth partFourth part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.00