In-vitro anticancer activity of Achyranthes aspera root extract against different human cancer cell lines

ABSTRACT Cancer is a public health problem all over the world. Developments in the treatment of cancer have lead to greatly improved survival and quality of life for the cancer patients by medicinal plants. The synthetic drugs are more toxic to animal body, whereas natural drugs play a dominant role in the pharmaceutical cure. Large number of plants and their isolated constituents has been shown to potential anticancer activity. 70% Ethanolic plant root extract of Achyranthes aspera L. showed in-vitro anticancer activity against different human cancer cell lines such as liver and colon. Sulforhodamine B dye (SRB) assay was done for in-vitro anticancer test assay. The in-vitro test was performed against two human cancer cell lines namely liver (Hep-2) & colon (HT-29). The activity was done using 100μg/ml of the root extract. It is confirmed that the plants root extract used for In-Vitro anti-cancer activity. In-Vitro experiments based on inhibition in the growth of the human cancer cells by the test sample. These observations clearly indicated that the Achyranthes aspera L. root extracts possess anticancer activity. Key Words: Cancer, Sulforhodamine B, Ethanolic, Liver, In-Vitro. REFERENCES Anand, P. Kunnumakkara B. Sundaram, C. Harikumar, KB. Tharakan, ST. Lai OS; Sung, B. Aggarwal, BB. 2008. Cancer is a preventable disease that requires major lifestyle changes. Pharm Res,; 25: 2097-2116 Arora, S. and Tandon, S. 2014. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest, The Scientific World Journal Volume (2014), Article ID 129697. Aziz, Md. Rahman, M. Mondal,A. Rahman, Md. 2005. 3-Acetoxy-6-benzoyloxyapangamide from Achyranthes aspera. J Pharm Sci;4(2):113-6. Bhoomika R.G, Shailaja G.M, and Ravindra G.M. 2007. Beneficial Effect of Achyranthes apsera Linn. In Toluene-Di-Isocyanate Induced Occupational Asthma in Rats. Global J Pharmacol,1(1):06-12. Duraipandiyan,V., N. A. Al-Harbi,Ignacimuthu,S. & Muthukumar,(2012).Antimicrobial activity of sesquiterpene lactones isolated from traditional medicinal plant, Costus speciosus (Koenex. Retz.) Sm.,"BMC Complementary and Alternative Medicine,12(13), pp. 2-6, Geetha, K. Narayanan, K. and Murugesan, A. 2010. Antimicrobial efficiency of Achyranthes aspera L. against selected pathogenic organisms. J Bio sci Res;1(3):187-90. Hullatti, KK. and Uma, DM. 2010. Activity Guided Isolation of Cytotoxic Compounds from Indian Medicinal Plants Using BSL Bioassay. JCPR;01:16-18. Jeetendra, YN. Deshmukh, VN. Shewale, V. and Narkhede M R. 2009. Aurangabadkar VM. Quantitation of oleanolic acid in Achyranthes aspera . Roots and leaves extracts by high-performance thin-layer chromatography. IJPRD -7. Moneim, AA. and Magdy, A. 2016. Review on Medicinal Plants as Potential Sources of Cancer Prevention and Treatment. European Journal of BiomedicaandPharmaceutical Sciences. Volume 3, Issue 6, 45-62. Monks. 1991. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines, J Natl.Cancer Institute 83 757-766. Mukherjee, P.K. 2008.Quality control of herbal drugs. Business Horizon Pharmaceutical Publishers, 13. Phillipson, JD. 2001. Phytochemistry and medicinal plants. Phytochemistry,56:237−243. Rama Mohan, RT. Karunakarb, B. and Vidyasagar, G. 2011. Seed Volatile Oil Analysis And Invitro Antimicrobial Activity Of Potential Medicinal Herb: Achyranthes Aspera. IJPT;3(1):1860-66. Rebucci ,M. and Michiels, C.( 2013). “Molecular aspects of cancer cell resistance to chemotherapy,” Biochemical Pharmacology, vol. 85, no. 9, pp. 1219–1226,View at Publisher-View at Google Scholar-View at Scopus Sciences, Volume 3, Issue 6, 45-62. Skehan, P.1990. New colorimetric cytotoxicity assay for anticancer drug screening, journal of National Cancer Institute 821107-1112. Verma, SK. Singh, S K. Singh, SS. and Mathur, A. 2010. In vitro cytotoxicity of Cannabis sativa and Trigonella foenum graecum against human cancer cell lines, Journal of Chemical and Pharmaceutical Research, 2 (4):861-865. Vijaya, K. Sankar, P. and Varatharajan, R. 2009. Anti-inflammatory activity of roots of Achyranthes aspera. Pharm Biol ;47(10):973–975. Sateesh Pujari, & Estari Mamidala. (2015). Anti-diabetic activity of Physagulin-F isolated from Physalis angulata fruits. The American Journal of Science and Medical Research, 1(2), 53–60. https://doi.org/10.5281/zenodo.7352308 Vijayan, Arun, Liju,V.B,. John J.V. Reena, B. Parthipan, C. and Renuka. (2007). Indian Journal of Traditional Knowledge, 6(4), 589-594. Wong, RSY. 2011. Apoptosis in cancer: from pathogenesis to treatment. Journal of Experimental and Clinical Cancer Research, vol. 30, no. 1, article 87