Production, Characterization and Kinetic Studies of Proteases by Screening of Indigenous Biomass

Proteases are vital industrial biocatalysts that form the largest group of enzymes acting as proteinases, peptidases, and amidases. Solid State fermentation (SSF) is recognized as an effective method to produce protease enzyme. The enzyme activity profile was obtained by performing enzyme assay showed that corn cobs gave maximum protease activity i.e., 53.297U/mL after seven days of incubation. Response surface methodology (RSM) was used to study the effect of different parameters on enzyme production. From the results inferred by RSM, the maximum enzyme activity was observed at 50°C, at pH 8.0 inoculated with 2mL inoculum size after seven days of incubation. KM and Vmax kinetic parameters were calculated 0.5219 mM and 12.19512 mM/min. The unique properties of protease enzyme such as high specificity, biodegradability and decreased the generation of waste materials makes it important enzyme for biotechnological applications