Antibacterial activity of methanolic extract of bitter leaf (Vernonia amygdalina) from various component fractions using column chromatography

This research work determined the antibacterial activities of various components at different ratios from column fractions of methanolic extract of bitter leaf (Vernonia amygdalina), meanwhile column chromatography been the best method of separating component nowadays. The column chromatography was perform using several type of solvent such as non-polar, semi-polar, polar solvent as well as their mixture in deference ratio. The phytochemical screening was carried out on the leaves extract of (Vernonia amygdalina) which revealed the presence of some active ingredients such as alkaloids, flavonoids, tannins, saponins, glycosides, cardiac glycoside and saponin glycosides in the extract, while anthraquinones and volatile oil were not identified. The research conclude that acetone- methanol 50:50 column fraction of methanolic extract of Vernonia amygdalina exhibits antibacterial activity against both gram positive and gram negative bacteria