Origin of bluetongue virus serotype 8 outbreak in Cyprus, September 2016

Table S1. Historical reference strains of BTV sequenced during this study and associated Genbank accession number for the genome segments. Figure S1. Phylogenetic trees were constructed for the coding regions of BTV a) VP1 protein (segment-1), b) VP3 protein (segment-3, c) NS1 protein (segment-5), d) VP5 protein (segment-6), and e) NS2 protein (segment-8). Maximum likelihood trees were constructed using IQ-Tree software [25] and the reliability of each tree was estimated by ultrafast bootstrap [26] analysis of 1000 replicates (bootstrap values of < 95% are not displayed). Figure S2. Aggregated trajectory densities of the forward NAME runs for three potential incursions of BTV infected midges to Cyprus. The figures show incursions with: a) a 24-hour flight time from sunset on 2016/09/07; b) a 36-hour flight time from sunrise on 2016/08/30; and c) a 24-hour flight time from sunset on 2016/08/20. Five sources are shown: 1 - northern Syria, 2 - southern Syria and northern Lebanon, 3 - central Lebanon, 4 - southern Lebanon and northern Israel, 5 - central Israel (as in Table 2)