EFFECT OF AZADIRACHTA INDICA A.JUSS. AND GYNURA PROCUMBENS (MERR.) LEAF EXTRACT COMBINATION TOWARDS FREE-RADICAL SCAVENGING ACTIVITY.

Introduction: The use of traditional medicine in Indonesia, especially those of herbal origin, is growing in response to the increasing price of the conventional medicine and the limited purchasing ability of the society. Extracted leaves from plants such as AzadirachtaindicaA.Juss. andGynuraprocumbens (Merr.) are examples of herbal medicine. The combination of both extract has the potential to be an antihyperglycemic agent through exploitation of its antioxidant properties. Aims & Objectives: This study focuses on testing the antioxidant activity of of a combination of ethanol extract AzadirachtaindicaA.Juss. andGynuraprocumbens (Merr.) by in vitro. Material & Methods: Antioxidant activity is tested using free radical DPPH (1,1-diphenyl-2-picrylhydrazyl) reduction method in comparison to vitamin C. The resulting antioxidant activity level of strength is determined based on IC50 values obtained. Results: antioxidant activity test showed that AzadirachtaindicaA.Juss. andGynuraprocumbens (Merr.) ethanol extract combination of 75:25 has the best IC50 value of 67.407 ?g/mL, while the 50:50 and 25:75 ethanol extract combination has IC50 value of 68.864 ?g/mL and 102.887 ?g/mL respectively. Discussion: The three combinations are weaker in comparison to the standard vitamin C antioxidant activity with IC50 value of 3.546 mg/mL. Statistical analysis using one-way ANOVA concludes significant difference in antioxidant activity of all three comparing groups. Conclusions:Gynuraprocumbens (Merr.) and Gynuraprocumbens (Merr.) extract combination of 75:25 has strong category antioxidant activity