The Study of Blood Vessels of Submandibular Gland and Urinary Bladder in Rats Using an Original Morphometric Analysis

The theoretical substantiation of the features of original algorithms for determining the radius of the lumen and the thickness of the vessel wall was provided. The features of using the algorithm for determining the wall thickness in order to calculate other indicators of hemocapillaries were indicated. The original computer software “VesselsMorpho Tools for ImageJ” was developed based on the algorithms mentioned above. It automatically determined a range of metric indicators of blood vessels. The most important among them were: the lumen area, lumen radius and lumen diameter, shape factor of the inner perimeter, total cross-sectional area of the vessel, wall thickness, Kernohan and Vohenvort indices, capillary basement membrane and BV transport zone thickness. This computer program reduces the time and is convenient and easy to use. The user only outlines the internal and external perimeters of BV, the outer contour of the lamina densa (on the electron microscope image of a capillary only) and the program automatically displays all indicators in table format and shows the result of measurements on photographs using different colours. More precise values of morphometric parameters of hemocapillaries of the submandibular gland and blood vessels of the mucous membrane of the urinary bladder being specific for one-year male Wistar rats were obtained