DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR CLOPIDOGREL BISULPHATE AND IRBESARTAN BY SIMULTANEOUS EQUATION SPECTROSCOPIC METHOD

Objective: The major approach take into consideration is to develop a simple, accurate, precise and reproducible method for development and validation of UV-visible spectrophotometric method for estimation of Clopidogrel Bisulphate and Irbesartan in synthetic mixture. Methods: In linearity spectra of the Clopidogrel Bisulphate and Irbesartan to shows to possible a simultaneous equation method but the zero order linearity spectra was converted to first derivative and second derivative spectroscopic method to not shows any zero crossing point so that this method was not possible. Results: In this spectroscopic method, for Clopidogrel Bisulphate 220 nm and 250 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 10-50 μg/ml at their respective λmax with correlation coefficient (r2) of 0.9996 and 0.9998 for Clopidogrel Bisulphate and Irbesartan, respectively. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (